Display panel, manufacturing method thereof and display device

The present disclosure provides a display panel, a manufacturing method thereof and a display device. The display panel includes a base substrate, a TFT array, a pixel definition layer, and a plurality of light-emitting units. The display panel further includes a plurality of spacers arranged on a surface of the pixel definition layer away from the base substrate, the plurality of spacers is formed integrally with the pixel definition layer, a surface of each spacer away from the base substrate includes a first portion and a second portion, a distance between the second portion and the base substrate is smaller than a distance between the first portion and the base substrate, and a ratio of a sum of areas of the first portions of the plurality of spacers to an area of a display region of the display panel is not smaller than a preset threshold.