Method of preparing a cryogenic sample with improved cooling characteristic

The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. A pair of conduits for transporting cryogenic fluid are provided, each of which conduits opens out into a mouthpiece, which mouthpieces are arranged...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kuijper, Maarten, Remigy, Hervé, Van Den Boogaard, Mathijs Petrus Wilhelmus, Hazenberg, John, Koh, Adrian, Spee, Ivanka, Van Der Does, Marius
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. A pair of conduits for transporting cryogenic fluid are provided, each of which conduits opens out into a mouthpiece, which mouthpieces are arranged to face each other across an intervening gap, wherein in said gap a sample that is provided on a substantially planar sample carrier can be received. Cryogenic fluid can be pumped through said conduits so as to concurrently flush from said mouthpieces and suddenly immerse the sample in cryogenic fluid from two opposite sides. As defined herein, at least one of said mouthpieces comprises at least two nozzle openings for evenly cooling said substantially planar sample carrier during said flushing.