Organelle genome modification using polynucleotide guided endonuclease

Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide...

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Bibliographische Detailangaben
Hauptverfasser: Sakai, Hajime, Kishore, Ganesh, Yoo, Byung-Chun, Orozco, Jr., Emil Meyer, Yadav, Narendra Singh
Format: Patent
Sprache:eng
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Zusammenfassung:Provided herein are methods and systems for altering the genome of an organelle. In some embodiments, the method comprises introducing into an organelle a recombinant DNA construct comprising a first polynucleotide encoding at least one guide RNA and a second polynucleotide encoding a polynucleotide guided polypeptide; and growing a cell comprising the organelle under conditions in which the first polynucleotide and the second polynucleotide are each expressed.