Single-use centrifuge containers for separating biological suspensions and methods of use

Single-use centrifuge containers for separating biological suspensions and methods of use

A method for separating a biological suspension includes dispensing a liquid suspension comprised of cells or microorganisms from a bioreactor or fermenter into a sterile compartment of a first bag assembly, the first bag assembly including a collapsible bag having of one or more sheets of flexible...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Ballhause, Norman, Tansey, III, Hugh H, Place, Ileana, Brown, Jason D, Hinz, Romana
Format: Patent
Sprache:eng
Schlagworte:
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL ORCHEMICAL PROCESSES
CENTRIFUGES
CHEMISTRY
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PERFORMING OPERATIONS
SPIRITS
TRANSPORTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A method for separating a biological suspension includes dispensing a liquid suspension comprised of cells or microorganisms from a bioreactor or fermenter into a sterile compartment of a first bag assembly, the first bag assembly including a collapsible bag having of one or more sheets of flexible film. The compartment of the first bag assembly is sealed closed. The first bag assembly, either with or without a manifold fluid coupled therewith, is then rotated, such as by using a centrifuge, so that the liquid suspension separates within the compartment into a pellet comprised of the cells or microorganisms and a liquid supernatant.