Methods and compositions for studying cell evolution

The subject matter disclosed herein is generally directed to methods and compositions for tagging cells of interest, tracking evolution of the tagged cells, and recovering the original tagged cells for further study. Specifically, cells are tagged with a DNA construct encoding a barcode sequence com...

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Bibliographische Detailangaben
Hauptverfasser: Beroukhim, Rameen, Johannessen, Cory, Tsai, Funien, Blainey, Paul, Bandopadhayay, Pratiti, Feldman, David
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The subject matter disclosed herein is generally directed to methods and compositions for tagging cells of interest, tracking evolution of the tagged cells, and recovering the original tagged cells for further study. Specifically, cells are tagged with a DNA construct encoding a barcode sequence comprising a guide sequence. Barcoded cells can then be recovered using a reporter construct having CRISPR target sequences specific for the cell having a barcode of interest.