Automatic dishwashing detergent composition comprising a protease

A phosphate-free automatic dishwashing cleaning composition comprising a protease wherein the protease has at least 80%, preferably at least 85%, more preferably at least 90% and especially at least 96% identity with the amino acid sequence of SEQ ID NO:1 or with the amino acid sequence of SEQ ID NO...

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Hauptverfasser: Ghirnikar, Roopa Santosh, Goedegebuur, Frits, Mulder, Harm, Van Stigt Thans, Sander, Souter, Philip Frank, Alekseyev, Viktor Yuryevich, Redestig, Nils Henning, Babe, Lilia Maria, Jackson, Michelle, Kaper, Thijs, Pickering, Carly, Garske, Adam, Dankmeyer, Lydia, Perez-Prat Vinuesa, Eva Maria, Pricelius, Sina, Stoner, Michael
Format: Patent
Sprache:eng
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Zusammenfassung:A phosphate-free automatic dishwashing cleaning composition comprising a protease wherein the protease has at least 80%, preferably at least 85%, more preferably at least 90% and especially at least 96% identity with the amino acid sequence of SEQ ID NO:1 or with the amino acid sequence of SEQ ID NO:2 and wherein the protease comprises amino acid substitutions selected from the group consisting of:(i) at least two amino acid substitutions selected from the group consisting of: X198G/A/K/L/Q/R/T/V/S/L, X207Q, X211Q/N and X212Q in combination with at least three amino acid substitutions selected from the group consisting of: X039E, X074D, X099R, X126A, X127E and X128G; or(ii) X039E-X074D-X099R-X116R-X126A-X127E-X128G-X211Q; X039E-X074D-X099R-X126A-X127E-X128G-X211N; X039E-X074D-X099R-X126A-X127E-X128G-X211Q; X039E-X074D-X099R-X126A-X127E-X128G-X207Q; or(iii) any of the proteases of (i) and (ii) further comprising at least one amino acid substitution selected from X242D and X256E; or(iv) X039E-X074D-X099R-X126A-X127E-X128G-X256E;using the SEQ ID NO:2 numbering.