Electrophoresis device
The present invention relates to apparatuses for use in electrophoretic separation of macromolecules and/or cells, and in which circulating buffer streams are not required for the electrophoretic separation of macromolecules and/or cells. In certain embodiments, the electrophoretic apparatus disclos...
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Zusammenfassung: | The present invention relates to apparatuses for use in electrophoretic separation of macromolecules and/or cells, and in which circulating buffer streams are not required for the electrophoretic separation of macromolecules and/or cells. In certain embodiments, the electrophoretic apparatus disclosed herein comprise a sample chamber and a harvest chamber separated by a size-exclusion membrane; non-circulating buffer chambers flanking each respective sample chamber and harvest chamber, wherein each buffer chamber is separated from each respective sample chamber and harvest chamber by an ion-permeable membrane (restriction membrane), and wherein the buffer chambers are sealed and contain a buffer solution; and an electrode positioned in each buffer chamber. Also disclosed are related methods of using the electrophoretic apparatus disclosed herein. |
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