Solid phase immunoassay apparatus and method for rapid detection of bacteria

An assay device is provided for use in determining the presence of a target analyte in a sample. The assay device comprises a solid platform comprising a fibrous mat, the solid platform impregnated with a first FRET chromophore. An antibody-FRET chromophore conjugate is immobilized on a surface of t...

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Bibliographische Detailangaben
Hauptverfasser:	Ajji, Abdellah, Heli, Bentolhoda
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING NANOTECHNOLOGY PERFORMING OPERATIONS PHYSICS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	An assay device is provided for use in determining the presence of a target analyte in a sample. The assay device comprises a solid platform comprising a fibrous mat, the solid platform impregnated with a first FRET chromophore. An antibody-FRET chromophore conjugate is immobilized on a surface of the solid platform, wherein the antibody-FRET chromophore conjugate comprises an antibody affixed to a second FRET chromophore. The first FRET chromophore and the second FRET chromophore are selected to provide an energy transfer from one to another when located within a Förster distance with respect to each other, thereby forming a FRET donor-acceptor chromophore pair. In a further aspect, a method of detecting a target analyte in a sample is provided. In yet a further aspect, packaging sheet materials and packaging articles employing the assay device under certain conditions are provided.