Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA

Variant DNA polymerases having improved properties and method for improved isothermal amplification of a target DNA

Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a...

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Bibliographische Detailangaben
Hauptverfasser: Tanner, Nathan, Potapov, Vladimir, Ong, Jennifer, Bei, Yanxia, Zhang, Yinhua
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
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Beschreibung
Zusammenfassung:Variants of the bacteriophage B103 DNA polymerase are described herein. The variant has improved properties, that include when compared to wild-type Phi29 DNA polymerase, at least one of the following: increased thermostability, improved reaction rate for DNA amplification, reduced background and a reduction of bias. Methods of using the DNA polymerase variant are also described herein.