Illumination for fluorescence imaging using objective lens

Illumination for fluorescence imaging using objective lens

A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Bowen, M. Shane, Condello, Danilo, Krumbuegel, Marco A, Liu, Aaron, Obradovic, Bojan, Nemiroski, Alex, Pratt, Mark, Dehlinger, Dietrich, Erie, Frederick, Hong, Stanley S, Lam, Anthony, Sun, Yinghua
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:A system includes: an objective lens; a first light source to feed first illuminating light through the objective lens and into a flowcell (e.g., with a relatively thin film waveguide) to be installed in the system, the first illuminating light to be fed using a first grating on the flowcell; and a first image sensor to capture imaging light using the objective lens, wherein the first grating is positioned outside a field of view of the first image sensor. Dual-surface imaging can be performed. Flowcells with multiple swaths bounded by gratings can be used. An auto-alignment process can be performed.