Alternating tangential flow rapid harvesting

Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The meth...

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Bibliographische Detailangaben
Hauptverfasser:	Bonham-Carter, John, Kudugunti, Shashi Kiran, Lin, WengLong Roy, Rusche, James R
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY BLASTING CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HEATING LIGHTING MECHANICAL ENGINEERING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL POSITIVE DISPLACEMENT MACHINES FOR LIQUIDS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS PUMPS PUMPS FOR LIQUIDS OR ELASTIC FLUIDS SEPARATION SPIRITS TRANSPORTING VINEGAR WEAPONS WINE
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Beschreibung
Zusammenfassung:	Methods and systems of harvesting a cell product from a cell culture by culturing cells in a fluid medium until the cells have produced a cell product at a harvest concentration are disclosed. The cells are cultured in a cell culture system including a bioreactor connected to an ATF device. The methods include draining fluid medium from the bioreactor through the outlet and the ATF device until the bioreactor volume reaches a predetermined volume, and the ATF column yields at an ATF outlet a liquid containing cell product and passes fluid medium with a concentration of cell product that is lower than the harvest concentration back into the bioreactor, extracting the liquid containing cell product from the ATF outlet, refilling the bioreactor with sterile phosphate buffered saline or fluid medium without any cell product, and repeating steps until a desired amount of cell product has been removed.