Primer set, kit and method for detecting two or more target nucleic acids

A primer set includes a terminal primer A including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5′ terminal sides,...

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Bibliographische Detailangaben
Hauptverfasser:	Nishizono, Takashi, Miyamoto, Shigehiko
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A primer set includes a terminal primer A including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a first target nucleic acid sequence, a k-th double-headed primer including two polynucleotides linked at their 5′ terminal sides, wherein one of the two polynucleotides includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a k-th target nucleic acid, and the other polynucleotide includes, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a complementary sequence of a (k+1)th target nucleic acid, and a terminal primer B including, in its 3′-terminal part, a nucleotide sequence that hybridizes to a 3′-terminal part of a nucleotide sequence of a N-th target nucleic acid.