Afucosylated protein, cell expressing said protein and associated methods

The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protei...

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Hauptverfasser:	Kamanagowda, Veeresha, Prasad, Bhargav, Dubey, Ashvini Kumar, Chakrabarty, Subhra Prakash, Thanigaivel, Aswini, Rodrigues, Kavitha Iyer, Mukunda, Pavithra, Nair, Karthika, Bhima Rao, Sahana, Bhattacharjee, Sanghamitra, Unnikrishnan, Divya, Murugesan, Sathyabalan, Dakshinamurthy, Pravin Kumar, Natarajan, Bairavabalakumar, Chatterjee, Sohang, Shenoy, Bharath Ravindra, Srinivasan, Sankaranarayanan, Bangalore Muniraju, Yogendra Manjunath, Sharma, Ankurina, Halan, Vivek, Maliwalave, Amol, Khan, Amir, Maity, Sunit, Hora, Anuradha, Ghosh, Maloy, Hazarika, Jahnabi
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protein. The resulting protein, specifically the resulting monoclonal antibody is completely afucosylated and reveals higher degree of antibody dependent cellular cytotoxicity.