Method for preparation of fungal mutant with high hydrolytic activity

Method for preparation of fungal mutant with high hydrolytic activity

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of Penicillium funiculosum. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in pa...

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Hauptverfasser: Sandhu, Simranjeet Kaur, Gupta, Ravi Prakash, Adsul, Mukund, Singhania, Reeta Rani, Mathur, Anshu Shankar, Puri, Suresh Kumar, Tuli, Deepak Kumar
Format: Patent
Sprache:eng
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BIOCHEMISTRY
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Zusammenfassung:A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of Penicillium funiculosum. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and β-glucosidase were 1.5 times higher than Penicillium funiculosum MRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.