Method for determining a protein-protein interaction

A method for determining an interaction between a first protein and a second protein comprises the steps of: expressing in a cell or introducing into a cell a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, and a second fusion protein comprisin...

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Bibliographische Detailangaben
Hauptverfasser:	Watanabe, Taku, Matsuzawa, Shun, Shoji, Toru
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for determining an interaction between a first protein and a second protein comprises the steps of: expressing in a cell or introducing into a cell a first fusion protein comprising the first protein, a multimerizable protein, and a fluorescent protein, and a second fusion protein comprising the second protein and a multimerizable protein; detecting a fluorescent focus formed by an association between the first fusion protein and the second fusion protein in the cell; and determining an interaction between the first protein and the second protein according to the detection of the fluorescent focus.