Systems and methods for generating a structured illumination image

A method for generating a composite image obtained in a fluorescence imaging system, comprising: determining the physical locations of a pinhole mask relative to a sample required to construct a full composite confocal image; generating in a control module a randomized order for the determined physi...

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Bibliographische Detailangaben
Hauptverfasser:	Boeschoten, Paul, Gunderson, Andrew, Rystrom, Larry, Gnehm, Chris, Lytle, Steven
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS SPECIALLY ADAPTED THEREFOR CINEMATOGRAPHY ELECTROGRAPHY HOLOGRAPHY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MATERIALS THEREFOR MEASURING OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS OPTICS ORIGINALS THEREFOR PHOTOGRAPHY PHOTOMECHANICAL PRODUCTION OF TEXTURED OR PATTERNED SURFACES,e.g. FOR PRINTING, FOR PROCESSING OF SEMICONDUCTORDEVICES PHYSICS TESTING
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Beschreibung
Zusammenfassung:	A method for generating a composite image obtained in a fluorescence imaging system, comprising: determining the physical locations of a pinhole mask relative to a sample required to construct a full composite confocal image; generating in a control module a randomized order for the determined physical locations; moving the photomask or the sample to the determined physical locations in the randomized order under control of the control module and using a translation stage; illuminating the sample through the photomask using an excitation light; capturing a plurality of images at each of the physical locations using a sensor in order to generate a set of data points; using the randomized order to generate a composite image based on the set of data points and measuring the brightness of at least some of the data points; and adjusting the brightness of some of the set of data points.