Binary vectors with minimized biosafety concerns and high transformation rates by engineered plant-derived transfer-DNA

An engineered plant-derived transfer (P-)DNA was designed and constructed based on a couple of T-DNA homologous sequences in sugar beet genome. Plant transformation efficacy of the engineered P-DNA was analyzed compared to conventional T-DNA in two independent systems, stable transformation of tobacco plant and sugar beet hairy roots. The outcomes demonstrated that plant transformation is directed well by vectors carrying the engineered P-DNA with higher efficiency than the conventional binary vector in both experimental systems. This vector was further improved by adding two matrix attachment regions within P-DNA. This new vector was even more efficient in gene transfer, higher than conventional binary vector.