Enzymatic encoding methods for efficient synthesis of large libraries

Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reactio...

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Bibliographische Detailangaben
Hauptverfasser:	Goldbech, Anne, Freskgard, Per-Ola, Gouliaev, Alex Haahr, Thisted, Thomas, Lundorf, Mikkel Dybro, Jakobsen, Søren Nyboe, Andersen, Anne Lee, Husemoen, Gitte Nystrup, Godskesen, Michael Anders, Dolberg, Johannes, Holtmann, Anette, de Leon, Daen, Jensen, Kim Birkebæk, Olsen, Eva Kampmann, Kaldor, Ditte Kievsmose, Sørensen, Anders Malling, Nørregaard-Madsen, Mads, Sams, Christian Klarner, Hansen, Anders Holm, Pedersen, Henrik, Glad, Sanne Schrøder, Neve, Søren, Franch, Thomas, Sløk, Frank Abildgaard, Petersen, Lene, Felding, Jakob, Kronborg, Tine Titilola Akinleminu
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).