Endpoint zygosity assay to detect Rf4 gene in maize

A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.

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Bibliographische Detailangaben
Hauptverfasser: Ren, Ruihua, Kumpatla, Siva Prasad, Zheng, Peizhong
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A method is provided for determining the zygosity of an Rf4 gene in a corn plant. A method may include performing a first PCR assay, a second PCR assay, quantifying probes used in the first and second PCR assays, and comparing the quantified probes to determine zygosity.