METHOD FOR ASSESSMENT OF ADENYL CYCLASE ACTIVITY IN ISOLATED BLOOD CELLS

The method for the assessment of adenyl cyclase activity in isolated blood cells comprises the reaction of the isolated blood cells in suspension with the inducer of adenyl cyclase activity. The standard system representing red blood cell suspension in the acidified isotonic saline solution of sodiu...

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Hauptverfasser:	NAHAICHUK VASYL IVANOVYCH, DEM''IANENKO VOLODYMYR VASYLIOVYCH
Format:	Patent
Sprache:	eng
Schlagworte:	DIAGNOSIS HUMAN NECESSITIES HYGIENE IDENTIFICATION MEDICAL OR VETERINARY SCIENCE PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SURGERY
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Beschreibung
Zusammenfassung:	The method for the assessment of adenyl cyclase activity in isolated blood cells comprises the reaction of the isolated blood cells in suspension with the inducer of adenyl cyclase activity. The standard system representing red blood cell suspension in the acidified isotonic saline solution of sodium chloride is used with this aim. The standardized suspension of red blood cells (optical density of 0.70) in a volume of 0.5 ml is added to the cuvette containing 20 ?l of adrenalin solution. The mixture is incubated for 2 min. Then 0.2 ml of hydrochloric acid solution (2.10-3 M/L) prepared in the isotonic saline solution of sodium chloride is added. The results of the assay are recorded photometrically using the self-recording unit connected with millivoltmeter.