METHOD FOR PRODUCING RIBONUCLEOTIDES
The method for producing ribonucleotides provides for hydrolysis of yeast ribonucleic acid in solution by pancreatic ribonuclease, precipitation by ethanol, ultrafiltration, treatment with 8-10-fold volume of ethanol, drying of the precipitate, its dissolution in sodium chloride solution, the steril...
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Zusammenfassung: | The method for producing ribonucleotides provides for hydrolysis of yeast ribonucleic acid in solution by pancreatic ribonuclease, precipitation by ethanol, ultrafiltration, treatment with 8-10-fold volume of ethanol, drying of the precipitate, its dissolution in sodium chloride solution, the sterilizing filtration, filling of the ampoules, and the sterilization. The hydrolysis is performed for 8-10 hours. The precipitation by ethanol is performed at the temperature of 8-12 DEGREE c. The precipitate is freeze-dried. The sterilization is carried out at a temperature of 110-112 DEGREE c for 8-10 minutes. |
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