Full flow-through process for purifying recombinant proteins

The present invention concerns a method for purifying a protein comprising in a continuous mode: one filtration step involving the use of at least one chelating agent, an exchanging step involving the use of at least one diafiltration membrane, and a polishing step involving the use of a combination...

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Bibliographische Detailangaben
Hauptverfasser: HEMET, CELINE, DUTHE, DIDIER, PEZZINI, JEROME, MOTHES, BENOIT
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The present invention concerns a method for purifying a protein comprising in a continuous mode: one filtration step involving the use of at least one chelating agent, an exchanging step involving the use of at least one diafiltration membrane, and a polishing step involving the use of a combination of membrane adsorbers, wherein two membrane adsorbers of said combination are orthogonal in terms of mechanism of action.