Method for making biomolecular microarray and spotting device

This invention provides a method for making a biomolecular microarray and spotting device for accurately spotting a solution containing probe biomolecules on probe biomolecule receiving solid phase areas, having a quantitative feature. A substrate having a surface with a plurality of probe biomolecule receiving solid phase areas (herein after as solid phase areas) for receiving uniquely and quantitatively is prepared, the solution containing the probe biomolecules is spotted on the solid phase areas, so as to fix the solution containing the biomolecules onto the solid phase areas (the spotting is so performed to cover the entire area of one solid phase area with one drop of the solution and one drop of the solution contains a number of the probe biomolecules which are more than one solid phase area can accommodate). The substrate has on its surface alignment marks for positioning the substrate, and the positions of the solid phase areas are determined in a positional relation with the alignment marks. The spotting of the solution is performed with respect to the solid phase areas whose positions are determined in a positional relation with the alignment marks.