Novel plasmids for plant transformation and method for using same

This invention relates to the agrobacterium mediated plant transformation, especially to transformation of plants with T-DNA, where read-through at the borders is prohibited. This can be done by inhibition of transfer of vector DNA to the plant cell by creating DNA binding sites outside the T-border...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	ELZINGA, NICOLAS, SIMONS, LAMBERTUS HENRICUS, STUIVER, MAARTEN HENDRIK, OHL, STEPHAN ANDREAS, GODDIJN, OSCAR JOHANNES MARIA+
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to the agrobacterium mediated plant transformation, especially to transformation of plants with T-DNA, where read-through at the borders is prohibited. This can be done by inhibition of transfer of vector DNA to the plant cell by creating DNA binding sites outside the T-borders, but also to insert a coding sequence outside the T-borders which, whether or not in cooperation with genes from the host plant or genes cotransferred, are toxic to plants so that there is counterselection for plants with superfluous vector-DNA.