Method for producing human chimeric antigen receptor T-cells enriched of stem cell-like memory T-cells

The present invention provides a method for producing human chimeric antigen receptor T-cell enriched of stem cell-like memory T-cells, comprising: (1) Providing human peripheral blood mononuclear cells (PBMC); (2) Human T cell isolation: adding magnetic beads coated with anti-CD3/CD28 antibodies to...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	LIN, XUAN-HUI, LAI, YI-HUI, LIN, CHEN-LUNG, LIN, CHIEN-TING, TUNG, TZU-HSUN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The present invention provides a method for producing human chimeric antigen receptor T-cell enriched of stem cell-like memory T-cells, comprising: (1) Providing human peripheral blood mononuclear cells (PBMC); (2) Human T cell isolation: adding magnetic beads coated with anti-CD3/CD28 antibodies to human PBMC to obtain human T cells/ magnetic beads complexes; (3) Human T cell activation: culturing the human T cells/ magnetic bead complex to obtain activated human T cells/ magnetic beads complexes; (4) Lentivirus transfection: adding lentivirus to the activated human T cells/ magnetic beads complexes to obtain human CAR-T cells/ magnetic beads complexes; (5) Magnetic beads removal: removing the magnetic beads from human CAR-T cells/ magnetic beads complexes after lentivirus transfection to obtain human CAR-T cells; and (6) human CAR-T cells expansion: further culturing the human CAR-T cells to obtain the human CAR-T cells enriched of stem cell-like memory T-cells. The present invention expands human T cells i