Ralstonia eutropha having low endotoxin content, and use thereof

Ralstonia eutropha having low endotoxin content, and use thereof

The present application belongs to the technical field of microorganisms and provides Ralstonia eutropha having low endotoxin content and a use thereof. Further provided is a use of the reduction of enzymatic activity and/or expression of the H16_A0228 protein and/or the H16_B0917 protein of Ralston...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: YIN, JIN, ZHANG, HAO-QIAN, FENG, JING, YANG, XIAO-YAN, LI, TENG
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present application belongs to the technical field of microorganisms and provides Ralstonia eutropha having low endotoxin content and a use thereof. Further provided is a use of the reduction of enzymatic activity and/or expression of the H16_A0228 protein and/or the H16_B0917 protein of Ralstonia eutropha in the reduction of the endotoxin content of Ralstonia eutropha. The inactivation of the H16_A0228 and H16_B0917 proteins of Ralstonia eutropha can markedly reduce the endotoxin content of Ralstonia eutropha. The engineered Ralstonia eutropha having loss-of-function of the H16_A0228 protein and H16_B0917 protein has significantly lowered endotoxin content, while cell dry weight and PHA yield are significantly raised. The present application provides new gene and strain resources for the development of engineer PHA strains, provides an effective method for reducing the endotoxin content of PHAs, and has important significance for extending the application of PHAs in medical materials.