Methods and systems to reduce auto-fluorescence in fluorescing samples

Exemplary sample processing methods are described that include providing an initial sample to a sample processing system. The sample processing system includes a light-emitting-diode, a temperature control unit, and a fluid supply unit. The methods also include irradiating the initial sample with li...

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Hauptverfasser: KIM, CHLOE, JOHNSON, JOSEPH R, GOH, CHRISTABELLE SI MEI, CHOI, CHANG H, LEE, YANG MING, TULU, USTUN SERDAR, CHENG, HSIAO-YING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:Exemplary sample processing methods are described that include providing an initial sample to a sample processing system. The sample processing system includes a light-emitting-diode, a temperature control unit, and a fluid supply unit. The methods also include irradiating the initial sample with light emitted from the light-emitting-diode to produce an irradiated sample. The methods may still further include adjusting a temperature of the irradiated sample with the temperature control unit to between 0 DEG C and 60 DEG C, and contacting the irradiated sample with a fluid from the fluid supply unit. The irradiated sample has a reduction in auto-fluorescence of greater than or about 50% compared to the initial sample. Exemplary sample processing systems are also described that include a light-emitting-diode, a temperature control unit, and a fluid supply unit.