Extraction method of Clinacanthus nutans, and use of it's extract for treating osteoporosis, neurodegenerative diseases, skeletal muscle atrophy and improving wound healing

The present invention provides a method for extracting Clinacanthus nutans, its extracts and its use for treating osteoporosis, neurodegenerative diseases, skeletal muscle atrophy and improving wound healing. Clinacanthus nutans was firstly extracted with ethanol and water, and partitioned between ethyl acetate and water to obtain a first aqueous layer and an ethyl acetate layer. The first aqueous layer was partitioned with n-butanol to obtain an n-butanol layerand a second aqueous layer. The ethyl acetate layer was portioned between ethanol aqueous solution and n-hexane to obtain a ethanol aqueous layer and an n-hexane layer.