Monitoring method for soil liquefaction based on microbial characteristics having a significant difference in the development and distribution of a microbial system compared with a soil sample without soil liquefaction

Monitoring method for soil liquefaction based on microbial characteristics having a significant difference in the development and distribution of a microbial system compared with a soil sample without soil liquefaction

This invention is a monitoring method for soil liquefaction based on microbial characteristics, which includes the following steps: (a) collecting a soil sample; (b) separating and collecting all DNA from the soil sample; (c) subjecting the DNA of the step (b) to a PCR reaction to generate a large n...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HSU, YIIUNG, CHAO, YING-TING
Format: Patent
Sprache:chi ; eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:This invention is a monitoring method for soil liquefaction based on microbial characteristics, which includes the following steps: (a) collecting a soil sample; (b) separating and collecting all DNA from the soil sample; (c) subjecting the DNA of the step (b) to a PCR reaction to generate a large number of sequences; (d) separately sequencing the sequences by using a sequencing method, and comparing same with a database and performing classification to form a plurality of operational classification units; and (e) performing [alpha]/[beta] discrepancy analysis, linear discriminant analysis, potency discrimination analysis and principal coordinate analysis to analyze the microflora composition of the soil sample. The sequenced sequences contain sequences of 16s rDNA regions V3-V4. If the soil sample is obtained from a soil liquefaction layer, the soil sample has a significant difference in the development and distribution of a microbial system compared with a soil sample without soil liquefaction.