Method for producing cis-unsaturated fatty acid by recombinant candida rugosa lipase 1 (rCRL1)

A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil/water mixture is provided, wherein the oil/water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant candida rugosa lipase 1 (rCRL...

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Hauptverfasser:	LEE, GUANIUN, CHEN, SHUN-NIANG, LIN, CHIAI, KUO, TINGUN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for producing cis-unsaturated fatty acid includes the operations below. (i) An oil/water mixture is provided, wherein the oil/water mixture includes 1 to 10 parts by weight of oil and 1 part by weight of water. (ii) 0.002 to 0.5 parts by weight of a recombinant candida rugosa lipase 1 (rCRL1) is added into the oil-water mixture. (iii) The oil/water mixture is emulsified. (iv) The emulsified oil-water mixture is hydrolyzed and fatty acid is generated. (v) Oil-water is separated at a temperature of 55 DEG C to 65 DEG C and an oil layer is extracted. (vi)The cooling and filtering step is performed to obtain cis-unsaturated fatty acid.