Method of preparing platelet lysate and use thereof for improving female pregnancy success rate

A method of preparing a platelet lysate and a use thereof are provided. The method includes the following steps. A collected whole blood is placed in a centrifuge tube containing a separation gel. A first centrifugation process is performed on the centrifuge tube containing the whole blood and the separation gel to separate the whole blood into a first upper portion, a middle portion, and a lower portion. The first upper portion and the middle portion are mixed to obtain a platelet-rich plasma fraction. The platelet-rich plasma fraction is clotted to obtain a platelet-rich fibrin gel. The platelet-rich fibrin gel is separated to obtain a platelet lysate.