Methods for purifying antibodies

The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography so...

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Bibliographische Detailangaben
Hauptverfasser:	MOLEK, JESSICA RACHEL, THOMSON, ANDREW S, YANCEY, KENNETH G, LEVY, NICHOLAS E, DUMETZ, ANDRE, GOKLEN, KENT E
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method of purifying a recombinant polypeptide from Host Cell Proteins (HCP), the method comprising: (a) applying a solution comprising the recombinant polypeptide and HCP to a superantigen chromatography solid support, (b) washing the superantigen chromatography solid support with a wash buffer comprising caprylate and arginine; and (c) eluting the recombinant polypeptide from the superantigen chromatography solid support, wherein the recombinant polypeptide is an anti-ICOS antibody or antigen binding fragment thereof.