Mutant nitrile hydratase, nucleic acid encoding the mutant nitrile hydratase, expression vector and transformant containing the nucleic acid, production method for muntant nitrile hydratase, and production method for amide compound

A mutant nitrile hydratase comprising an [alpha]-subunit and a [beta]-subunit, wherein the mutant nitrile hydratase includes amino acid substitution of at least one amino acid residue of at least one position selected from 40th and 43rd positions from the N-terminal of the [alpha]-subunit and 205th,...

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Bibliographische Detailangaben
Hauptverfasser:	KAI, KEIICHIROU, TOKUDA, JUNKO, TATENO, TOSHIHIRO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A mutant nitrile hydratase comprising an [alpha]-subunit and a [beta]-subunit, wherein the mutant nitrile hydratase includes amino acid substitution of at least one amino acid residue of at least one position selected from 40th and 43rd positions from the N-terminal of the [alpha]-subunit and 205th, 206th and 215th positions from the N-terminal of the [beta]-subunit, by at least one specific amino acid residue, in a Pseudonocardia Thermophila-derived nitrile hydratase.