Method for detecting and determining quantity of galacto-oligosaccharides

A method is provided for simply and inexpensively separating 4'-GL from a sample including galacto-oligosaccharides and dextrins and accurately determining the quantity of 4'-GL and galacto-oligosaccharides. A method for detecting and determining the quantity of galacto-oligosaccharides in...

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Bibliographische Detailangaben
Hauptverfasser: MIZUKOSHI, HARUMI, KIMURA, KAZUMASA
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:A method is provided for simply and inexpensively separating 4'-GL from a sample including galacto-oligosaccharides and dextrins and accurately determining the quantity of 4'-GL and galacto-oligosaccharides. A method for detecting and determining the quantity of galacto-oligosaccharides in a sample including galacto-oligosaccharides and dextrins, said method for detecting and determining the quantity of galacto-oligosaccharides being characterized in that the sample is made to react with a derivatization reagent, the dextrins and galacto-oligosaccharides in the sample are derivatized, and then the galacto-oligosaccharide components in the sample are separated through high-performance liquid chromatography using a C30 reversed-phase chromatography column.