Method for obtaining binding kinetic rate constants using fiber optics particle plasmon resonance (FOPPR) sensor

A method for obtaining the binding kinetic rate constants using fiber optic particle plasmon resonance (FOPPR) sensor, suitable for a test solution with two or more concentrations, which employs the following major steps: providing one FOPPR sensor instrument system, obtaining optical signal intensities at the initial time and the steady state signal intensities of the two or more regions in the intensity versus time curve separately, substituting the measured signal intensity values into the formula which is derived using pseudo-first order rate equation model. In addition, this method measures the temporal signal intensity evolution under static conditions as the samples are quickly loaded. As a result, unlike the conventional device where the sample is continuously infused, the method is able to measure the association and dissociation rate constants of which the upper bound are not limited by the sample flow rate.