DNA PPR-IL 2-19 RECOMBINATION PLASMID WHICH CODES HUMAN INTERLEUCINE-2 SYNTHESIS AND STRUCTURE ESCHERICHIA COLI STRAIN, HUMAN INTERLEUCINE-2 PRODUCENT

A recombinant plasmid DNA pPR-IL2-19, coding for synthesis of human interleukin-2 has a size of 3.85 k.b.p. and consists of the following elements: BamHI-BglII, a fragment of the size of 3.4 k.b.p. of vector plasmid pPR124B obtained on the basis of pPR40 and pML24; Cfr13-BglII, a fragment of the siz...

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Hauptverfasser:	TSIMANIS ALEKSANDR YU,SU, DEBABOV VLADIMIR G,SU, CHERNOVSKAYA TATYANA V,SU, YURIN VITALIJ L,SU, KOSTROV SERGEJ V,SU, MOCHULSKIJ ANDREJ V,SU, GREN ELMAR YA,SU, STRONGIN ALEKSANDR YA,SU, STERKIN VIKTOR E,SU, KOZLOV YURIJ I,SU, TRUKHAN MAKSIM E,SU, SKVORTSOVA MARINA A,SU, MAZEL SVETLANA M,SU, ROMANCHIKOVA NADEZHDA V,SU, KOSIKOV ALEKSANDR I,SU, AVOT ANDRIS YA,SU, MASHKO SERGEJ V,SU, NOSOVSKAYA ELENA A,SU
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Zusammenfassung:	A recombinant plasmid DNA pPR-IL2-19, coding for synthesis of human interleukin-2 has a size of 3.85 k.b.p. and consists of the following elements: BamHI-BglII, a fragment of the size of 3.4 k.b.p. of vector plasmid pPR124B obtained on the basis of pPR40 and pML24; Cfr13-BglII, a fragment of the size of 0.45 k.b.p. of plasmid pAA1213-23B obtained by modifying plasmid pAA1213-23 constructed on the basis of plasmid pBR-322 containing the kDNA-copy of the gene of human interleukin-2. A method of construction of the above recombinant plasmid DNA consists in obtaining, by modifying plasmid pAA1213-23, plasmid pAA1213-23B, a fragment of which, together with the gene of interleukin-2, is integrated into vector plasmid pPR-IL2-19, and in transforming, by the thus obtained mixture, cells of E. coli C600 and in separating plasmid pPR-IL2-19 from the clones grown on a selective medium. A strain of bacteria E. coli VNIIGENETIKA VL 903 (pPR-IL2-19) as producer of human interleukin-2 deposited on 12.01.87 under No. 1869 at the culture collection of the USSR Research Institute for Antibiotics.