Sequence-specific DNA recombination in eukaryotic cells

The invention relates to a method for carrying out the sequence-specific recombination of DNA in eukaryotic cells comprising the following steps: inserting a first DNA sequence into a cell, inserting a second DNA sequence into a cell, and carrying out the sequence-specific recombination using the ac...

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Bibliographische Detailangaben
Hauptverfasser:	LORBACH ELKE, DROGE PETER, CHRIST NICOLE
Format:	Patent
Sprache:	eng ; slo
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for carrying out the sequence-specific recombination of DNA in eukaryotic cells comprising the following steps: inserting a first DNA sequence into a cell, inserting a second DNA sequence into a cell, and carrying out the sequence-specific recombination using the action of a bacteriophage lambda integrase Int. A preferred embodiment of the invention relates to a method that additionally comprises the step of carrying out a second sequence-specific recombination of DNA using the action of an Int and of an Xis factor. The invention also relates to vectors and to their use as medicaments. Je opísaný spôsob sekvenčne špecifickej rekombinácie DNA v eukaryotických bunkách, ktorý zahrnuje zavedenie prvej DNA sekvencie do bunky, zavedenie druhej DNA sekvencie do bunky a vykonanie sekvenčnešpecifickej rekombinácie pomocou integrázy Int baktériofága lambda. Výhodne je opísaný spôsob, ktorý ďalej zahrnuje vykonanie sekvenčne špecifickej rekombinácie DNA pomocou Int a Xis faktora. Predkladaný vynález sa ďalej týka vektorov a ich použitiaako liečiv.