METHOD FOR EXTRACTION OF PENICILLIN AMIDOHYDROLASE FROM CELLS OF MICROORGANISMS
Penicillin amido hydrolase is extracted from the microorganisms cells by the effect of non-ionic tensides, from the line of alkyphenyl polyethylene glycoethers or in combination with guanidine and its O- and S- analogues and ethylene diamine tetraacetic acid or its salt under the temperature 4 - 50...
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| creator | WELWARD LADISLAV MICHALKOVA EVA GASPAR ROMAN |
| description | Penicillin amido hydrolase is extracted from the microorganisms cells by the effect of non-ionic tensides, from the line of alkyphenyl polyethylene glycoethers or in combination with guanidine and its O- and S- analogues and ethylene diamine tetraacetic acid or its salt under the temperature 4 - 50 C during 0.5 to 24 hours. These substances are added to aqueous suspensions of the cells in amounts that their concentrations in suspension were 0.1 - 2 % wt., 0.05 - 1.0 mol.dm(exp)-3, resp. 0.01 - 0.1 mol.dm(exp)-3. Under the said conditions penicillin amidohydrolase is extracted from the cell structures to the outer environment with the high activity and extract. |
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These substances are added to aqueous suspensions of the cells in amounts that their concentrations in suspension were 0.1 - 2 % wt., 0.05 - 1.0 mol.dm(exp)-3, resp. 0.01 - 0.1 mol.dm(exp)-3. Under the said conditions penicillin amidohydrolase is extracted from the cell structures to the outer environment with the high activity and extract.</description><edition>6</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PERFORMING OPERATIONS ; PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SEPARATION ; SPIRITS ; TRANSPORTING ; VINEGAR ; WINE</subject><creationdate>1999</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19990507&DB=EPODOC&CC=SK&NR=149097A3$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19990507&DB=EPODOC&CC=SK&NR=149097A3$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>WELWARD LADISLAV</creatorcontrib><creatorcontrib>MICHALKOVA EVA</creatorcontrib><creatorcontrib>GASPAR ROMAN</creatorcontrib><title>METHOD FOR EXTRACTION OF PENICILLIN AMIDOHYDROLASE FROM CELLS OF MICROORGANISMS</title><description>Penicillin amido hydrolase is extracted from the microorganisms cells by the effect of non-ionic tensides, from the line of alkyphenyl polyethylene glycoethers or in combination with guanidine and its O- and S- analogues and ethylene diamine tetraacetic acid or its salt under the temperature 4 - 50 C during 0.5 to 24 hours. These substances are added to aqueous suspensions of the cells in amounts that their concentrations in suspension were 0.1 - 2 % wt., 0.05 - 1.0 mol.dm(exp)-3, resp. 0.01 - 0.1 mol.dm(exp)-3. 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These substances are added to aqueous suspensions of the cells in amounts that their concentrations in suspension were 0.1 - 2 % wt., 0.05 - 1.0 mol.dm(exp)-3, resp. 0.01 - 0.1 mol.dm(exp)-3. Under the said conditions penicillin amidohydrolase is extracted from the cell structures to the outer environment with the high activity and extract.</abstract><edition>6</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE |
| title | METHOD FOR EXTRACTION OF PENICILLIN AMIDOHYDROLASE FROM CELLS OF MICROORGANISMS |
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