METHOD FOR EXTRACTION OF PENICILLIN AMIDOHYDROLASE FROM CELLS OF MICROORGANISMS

Penicillin amido hydrolase is extracted from the microorganisms cells by the effect of non-ionic tensides, from the line of alkyphenyl polyethylene glycoethers or in combination with guanidine and its O- and S- analogues and ethylene diamine tetraacetic acid or its salt under the temperature 4 - 50...

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Bibliographische Detailangaben
Hauptverfasser:	WELWARD LADISLAV, MICHALKOVA EVA, GASPAR ROMAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Penicillin amido hydrolase is extracted from the microorganisms cells by the effect of non-ionic tensides, from the line of alkyphenyl polyethylene glycoethers or in combination with guanidine and its O- and S- analogues and ethylene diamine tetraacetic acid or its salt under the temperature 4 - 50 C during 0.5 to 24 hours. These substances are added to aqueous suspensions of the cells in amounts that their concentrations in suspension were 0.1 - 2 % wt., 0.05 - 1.0 mol.dm(exp)-3, resp. 0.01 - 0.1 mol.dm(exp)-3. Under the said conditions penicillin amidohydrolase is extracted from the cell structures to the outer environment with the high activity and extract.