MONOCLONAL ANTIBODIES VERSUS SOLUBLE TNF-ALPHA RECEPTORS P55 AND P75 AND TNF-ALPHA AND ITS ANALOGUES

The submitted invention belongs to the field of pharmaceutical industry and refers to new monoclonal antibodies and their fragments which bind to an accessible surface of human recombinant TNF-Alpha, TNF-Alpha analogues and TNF-Alpha soluble receptors. The invention also refers to stable cell lines...

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Hauptverfasser:	MENART VIKTOR, ZUNEC PETRA, CURIN-SERBEC VLADKA, GABERC - POREKAR VLADIMIRA, SCHUURBIERS ANNETTE, STALC ANTON
Format:	Patent
Sprache:	eng ; slv
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The submitted invention belongs to the field of pharmaceutical industry and refers to new monoclonal antibodies and their fragments which bind to an accessible surface of human recombinant TNF-Alpha, TNF-Alpha analogues and TNF-Alpha soluble receptors. The invention also refers to stable cell lines of hybridomas which are capable of producing such monoclonal antibodies, to the procedure for their preparation and to pharmaceutical preparations which contain them. Further, the invention refers to the method for a quantitative determination of a human TNF-Alpha, free and complex with soluble TNF-Alpha receptors p55 and p75 as well as putty which is used in this procedure. It was not possible to simultaneously determine free TNF-Alpha macromolecules and their soluble receptors as well as their complexes by means of monoclonal antibodies known so far and the reagents developed from them. This invention also refers to TNF-Alpha analogue TNF-Alpha His 107/108, Cys95/148 which is used in the complex with TNF-Alpha receptors as an antigen for the immunization of mice. Predlozeni izum spada v podrocje farmacevtske industrije in se nanasa na novamonoklonska protitelesa in njihove fragmente, ki se vezejo na dostopno povrsino humanega rekombinantnega TNF-Alfa, TNF-Alfa analogov in TNF-Alfa topnih receptorjev. Izum se nanasa se na stabilne celicne linije hibridomov, ki so sposobne taka monoklonska protitelesa proizvajati, postopek za njihovo pripravo in farmacevtske pripravke, ki jih vsebujejo. Nadalje se izum nanasa tudi na metodo za kvantitativno dolocanje humanega TNF-Alfa, prostega in kompleksnega s topnima TNF-Alfa receptorjema p55 in p75 ter kit, ki se pri tem uporablja. Z do sedaj poznanimi monokonskimi protitelesi in iz njih razvitimi reagenti ni bilo mozno istocasno dolocati tako prostih makromolekul TNF-Alfa in njihovih topnih receptorjev kot tudi njihovih kompleksov. Predmet tega izuma je tudi TNF-Alfa analog TNF-Alfa His107/108, Cys95/148, ki ga v kompleksu s TNF-Alfa receptorjema uporabljamo kot antigen za imunizacijo misi.