HAEMOPLASMA DETECTION METHOD

A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of: i) treating said sample to release bacterial nucleic acid in the sample, ii) amplifying a segment of a 16S rRNA gene by real-t...

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Hauptverfasser: ROSENGARTEN Renate, ZIMMERMAN Carl-Ulrich Richard
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creator ROSENGARTEN Renate
ZIMMERMAN Carl-Ulrich Richard
description A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of: i) treating said sample to release bacterial nucleic acid in the sample, ii) amplifying a segment of a 16S rRNA gene by real-time PCR (qPCR) using an oligonucleotide primer pair, said oligonucleotide primer pair consisting of a forward primer comprising at least 10 consecutive bases of SEQ ID 1 and a reverse primer comprising at least 10 consecutive bases of SEQ ID 2, thereby producing an amplification product, and iii) detecting the amplification product by means producing a detection signal correlating with the amount of the amplification product which is indicative of any haemoplasma species within the genus Mycoplasma; and a composition comprising the forward primer and the reverse primer.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
title HAEMOPLASMA DETECTION METHOD
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