HAEMOPLASMA DETECTION METHOD
A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of: i) treating said sample to release bacterial nucleic acid in the sample, ii) amplifying a segment of a 16S rRNA gene by real-t...
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creator | ROSENGARTEN Renate ZIMMERMAN Carl-Ulrich Richard |
description | A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of:
i) treating said sample to release bacterial nucleic acid in the sample,
ii) amplifying a segment of a 16S rRNA gene by real-time PCR (qPCR) using an oligonucleotide primer pair, said oligonucleotide primer pair consisting of a forward primer comprising at least 10 consecutive bases of SEQ ID 1 and a reverse primer comprising at least 10 consecutive bases of SEQ ID 2, thereby producing an amplification product, and
iii) detecting the amplification product by means producing a detection signal correlating with the amount of the amplification product which is indicative of any haemoplasma species within the genus Mycoplasma; and a composition comprising the forward primer and the reverse primer. |
format | Patent |
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i) treating said sample to release bacterial nucleic acid in the sample,
ii) amplifying a segment of a 16S rRNA gene by real-time PCR (qPCR) using an oligonucleotide primer pair, said oligonucleotide primer pair consisting of a forward primer comprising at least 10 consecutive bases of SEQ ID 1 and a reverse primer comprising at least 10 consecutive bases of SEQ ID 2, thereby producing an amplification product, and
iii) detecting the amplification product by means producing a detection signal correlating with the amount of the amplification product which is indicative of any haemoplasma species within the genus Mycoplasma; and a composition comprising the forward primer and the reverse primer.</description><language>eng ; slv</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210430&DB=EPODOC&CC=SI&NR=3438280T1$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,309,781,886,25566,76549</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20210430&DB=EPODOC&CC=SI&NR=3438280T1$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>ROSENGARTEN Renate</creatorcontrib><creatorcontrib>ZIMMERMAN Carl-Ulrich Richard</creatorcontrib><title>HAEMOPLASMA DETECTION METHOD</title><description>A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of:
i) treating said sample to release bacterial nucleic acid in the sample,
ii) amplifying a segment of a 16S rRNA gene by real-time PCR (qPCR) using an oligonucleotide primer pair, said oligonucleotide primer pair consisting of a forward primer comprising at least 10 consecutive bases of SEQ ID 1 and a reverse primer comprising at least 10 consecutive bases of SEQ ID 2, thereby producing an amplification product, and
iii) detecting the amplification product by means producing a detection signal correlating with the amount of the amplification product which is indicative of any haemoplasma species within the genus Mycoplasma; and a composition comprising the forward primer and the reverse primer.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZJDxcHT19Q_wcQz2dVRwcQ1xdQ7x9PdT8HUN8fB34WFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFicmpeakl8cGexibGFkYWBiEhhsbEqAEAfjMhSQ</recordid><startdate>20210430</startdate><enddate>20210430</enddate><creator>ROSENGARTEN Renate</creator><creator>ZIMMERMAN Carl-Ulrich Richard</creator><scope>EVB</scope></search><sort><creationdate>20210430</creationdate><title>HAEMOPLASMA DETECTION METHOD</title><author>ROSENGARTEN Renate ; ZIMMERMAN Carl-Ulrich Richard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_SI3438280TT13</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng ; slv</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>ROSENGARTEN Renate</creatorcontrib><creatorcontrib>ZIMMERMAN Carl-Ulrich Richard</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>ROSENGARTEN Renate</au><au>ZIMMERMAN Carl-Ulrich Richard</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>HAEMOPLASMA DETECTION METHOD</title><date>2021-04-30</date><risdate>2021</risdate><abstract>A method for detecting at least one haemoplasma species within the group of haemoplasmas of the genus Mycoplasma in a biological sample, said method comprising the steps of:
i) treating said sample to release bacterial nucleic acid in the sample,
ii) amplifying a segment of a 16S rRNA gene by real-time PCR (qPCR) using an oligonucleotide primer pair, said oligonucleotide primer pair consisting of a forward primer comprising at least 10 consecutive bases of SEQ ID 1 and a reverse primer comprising at least 10 consecutive bases of SEQ ID 2, thereby producing an amplification product, and
iii) detecting the amplification product by means producing a detection signal correlating with the amount of the amplification product which is indicative of any haemoplasma species within the genus Mycoplasma; and a composition comprising the forward primer and the reverse primer.</abstract><oa>free_for_read</oa></addata></record> |
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subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
title | HAEMOPLASMA DETECTION METHOD |
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