ENZYMATIC ENCODING METHODS FOR EFFICIENT SYNTHESIS OF LARGE LIBRARIES

Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reactio...

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Bibliographische Detailangaben
Hauptverfasser:	DOLBERG Johannes, GLAD Sanne Schroder, THISTED Thomas, SORENSEN Anders Malling, HANSEN Anders Holm, KALDOR Ditte Kievsmose, FRANCH Thomas, HUSEMOEN Gitte Nystrup, SLOK Frank Abildgaard, FELDING Jakob, HOLTMANN Anette, SAMS Christian Klarner, PEDERSEN Henrik, OLSEN Eva Kampmann, JENSEN Kim Birkebaek, NORREGAARD-MADSEN Mads, JAKOBSEN Soren Nyboe, FRESKGARD Per-Ola, PETERSEN Lene, NEVE Soren, GODSKESEN Michael Anders, GOULIAEV Alex Haahr, ANDERSEN Anne Lee, DE LEON Dean, KRONBERG Tine Titiola Akinleminu, LUNDORF Mikkel Dybro, GOLDBECH Anne
Format:	Patent
Sprache:	eng ; slv
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed is a method for obtaining a bifunctional complex comprising a molecule linked to a single stranded identifier oligonucleotide, wherein a nascent bifunctional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is a) reacted at the chemical reaction site with one or more reactants, and b) reacted enzymatically at the priming site with one or more tag(s) identifying the reactant(s).