LARGE SCALE DISPOSABLE BIOREACTOR

The present invention concerns a disposable device for culturing and harvesting plant tissue and/or cells comprising a non-rigid container having a volume of at least 400 liters and being configured with gas exchange ports and a harvesting port enabling said device to be used continuously for at least two consecutive culturing/harvesting cycles, wherein the gas exchange ports include a plurality of gas inlet ports positioned on a surface of the container within the bottom one half of a height of the container configured to mix and aerate medium in the container, wherein the container is formed with an inverted conical lower portion and wherein said gas inlet ports are positioned on the conical lower portion, wherein the device has neither mechanical agitation to mix the medium in the container nor a sparger to distribute gas when medium is present therein and wherein the device is designed and constructed for maintaining oxygen saturation and shear forces suitable for culturing said plant tissue and/or cells.