DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION

Disclosed are mutant DNA polymerases having increased 3 '-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acid...

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Bibliographische Detailangaben
Hauptverfasser:	BAUER, KEITH, A, MYERS, THOMAS, W, REICHERT, FRED, L, SAN FILIPPO, JOSEPH, SCHOENBRUNNER, NANCY, J
Format:	Patent
Sprache:	eng
Online-Zugang:	Volltext bestellen
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Beschreibung
Zusammenfassung:	Disclosed are mutant DNA polymerases having increased 3 '-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.