PROCESS

PROCESSAbstractA process is provided for the preparation of a highly pure albumin solution theprocess comprising subjecting albumin (preferably expressed and secreted bytransformed yeast) to a series of chromatographic steps. Preferably, the processcomprises the steps of positive mode cation exchang...

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Hauptverfasser:	MICHEL GASTON JOSEPH GRANDGEGORGE, HENDRIK VAN URK, PHILIP HARVEY MORTON, JOHN RODNEY WOODROW, DAVID JOHN MEAD, JEAN-LUC BERNARD VERON, DARRELL SLEEP, DAVID JAMES BALLANCE, STEPHEN BEREZENKO, JASON CAMERON, ANDREW JOHN CARTWRIGHT
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	PROCESSAbstractA process is provided for the preparation of a highly pure albumin solution theprocess comprising subjecting albumin (preferably expressed and secreted bytransformed yeast) to a series of chromatographic steps. Preferably, the processcomprises the steps of positive mode cation exchange chromatography, positive modeanion exchange chromatography, positive mode affinity chromatography, negativemode affinity chromatography (preferably using immobilised aminophenylboronicacid), negative mode cation exchange chromatography, and negative or positive modeanion exchange chromatography. A process for reducing the level of nickel in analbumin solution is also disclosed, as is a recombinant albumin coding sequencecomprising two or more in-frame translation stop codons. Also disclosed is a processfor producing recombinant albumin, the process comprising culturing a fungal cellexpressing a recombinant albumin coding sequence, wherein the cell has a reducedcapacity of mannosylation of the recombinantly-expressed albumin.Figure 8