A DETECTION METHOD

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property 1111111111111111111111111111111111111101011101111111111010H0111110111111111111 Organization International Bureau (10) International Publication Number (43) International Publication Date ...0\" WO 2018/174830 Al 27 September 2018 (27.09.2018) W I POI PCT (51) International Patent Classification: crobiology, and Immunology, Science Drive 2, Singapore C12Q 1/6851 (2018.01) A61P 31/14 (2006.01) 117545 (SG). NYO, MM; c/o National University of Sin- A61K 31/7088 (2006.01) gapore, Blk MD4, Level 3, 5, Department of Microbiology, (21) International Application Number: and Immunology, Science Drive 2, Singapore 117545 (SG). PCT/SG2018/050134 (74) Agent: AMICA LAW LLC; 30 Raffles Place, #14-01 (22) International Filing Date: Chevron House, Singapore 048622 (SG). 26 March 2018 (26.03.2018) (81) Designated States (unless otherwise indicated, for every (25) Filing Language: English kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, (26) Publication Language: English CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, (30) Priority Data: DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, 10201702437X 24 March 2017 (24.03.2017) SG KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, (71) Applicants: AGENCY FOR SCIENCE, TECHNOLO- MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, GY AND RESEARCH [SG/SG]; 1 Fusionopolis Way, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, #20-10 Connexis, Singapore 138632 (SG). NATIONAL SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, UNIVERSITY OF SINGAPORE [SG/SG]; 21 Lower TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. Kent Ridge Road, Singapore 119077 (SG). (84) Designated States (unless otherwise indicated, for every (72) Inventors: CHU, Jang Hann, Justin; c/o National Univer- kind of regional protection available): ARIPO (BW, GH, sity of Singapore, Blk MD4, Level 3, 5, Department of Mi- GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, = = (54) Title: A DETECTION METHOD = 20- - _ 15- = c = = - w = 0- = = .. . -- . ' \ k., `N * * LI HFMD Healthy = -5 * * * * * . * * = tit2 f o .Q b 4 2 faQ 0 . 4 2 n a 4 2 42 toQ tiF t 4 1 01' 01 1 4 1 et. N* , i,b 1* eb 41 * 0 *,,.;* A T 4 1: A T A ,,, scr A T Ak *ivr A T A ,,, *icr ..- i .- , .- .- 74 V :N - _ I * 0 0 0 0 0- 0 0- r y a ry ry a a ry a ry a 0 s'% co `''*