GENETIC CONSTRUCT BASED ON CRISPR/CAS9 GENOME EDITING SYSTEM ENCODING CAS9 NUCLEASE FOR GENOME EDITING OF MONOCOTYLEDONOUS GRAIN CROPS BASED ON DOUBLE SELECTION OF PLANTS

FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, particularly, to a genetic construct based on the CRISPR/Cas9 genome editing system encoding the Cas9 nuclease, containing the bar gene-selectable marker and the gfp reporter gene.EFFECT: invention ensures efficient sel...

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Hauptverfasser: Shulga Olga Albertovna, Klementeva Anna Aleksandrovna, Miroshnichenko Dmitrij Nikolaevich, Timerbaev Vadim Rafailovich, Dolgov Sergej Vladimirovich
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creator Shulga Olga Albertovna
Klementeva Anna Aleksandrovna
Miroshnichenko Dmitrij Nikolaevich
Timerbaev Vadim Rafailovich
Dolgov Sergej Vladimirovich
description FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, particularly, to a genetic construct based on the CRISPR/Cas9 genome editing system encoding the Cas9 nuclease, containing the bar gene-selectable marker and the gfp reporter gene.EFFECT: invention ensures efficient selection of modified plants without additional molecular and biological testing.1 cl, 6 dwg, 2 ex Изобретение относится к области биотехнологии, в частности к генетической конструкции на основе системы редактирования генома CRISPR/Cas9, кодирующей нуклеазу Cas9, содержащей ген-селективный маркер bar и репортерный ген gfp. Изобретение обеспечивает эффективный отбор модифицированных растений без дополнительных молекулярно-биологических тестов. 6 ил., 2 пр.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title GENETIC CONSTRUCT BASED ON CRISPR/CAS9 GENOME EDITING SYSTEM ENCODING CAS9 NUCLEASE FOR GENOME EDITING OF MONOCOTYLEDONOUS GRAIN CROPS BASED ON DOUBLE SELECTION OF PLANTS
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