GENETIC CONSTRUCT BASED ON CRISPR/CAS9 GENOME EDITING SYSTEM ENCODING CAS9 NUCLEASE FOR GENOME EDITING OF MONOCOTYLEDONOUS GRAIN CROPS BASED ON DOUBLE SELECTION OF PLANTS

FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, particularly, to a genetic construct based on the CRISPR/Cas9 genome editing system encoding the Cas9 nuclease, containing the bar gene-selectable marker and the gfp reporter gene.EFFECT: invention ensures efficient sel...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	Shulga Olga Albertovna, Klementeva Anna Aleksandrovna, Miroshnichenko Dmitrij Nikolaevich, Timerbaev Vadim Rafailovich, Dolgov Sergej Vladimirovich
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology, particularly, to a genetic construct based on the CRISPR/Cas9 genome editing system encoding the Cas9 nuclease, containing the bar gene-selectable marker and the gfp reporter gene.EFFECT: invention ensures efficient selection of modified plants without additional molecular and biological testing.1 cl, 6 dwg, 2 ex Изобретение относится к области биотехнологии, в частности к генетической конструкции на основе системы редактирования генома CRISPR/Cas9, кодирующей нуклеазу Cas9, содержащей ген-селективный маркер bar и репортерный ген gfp. Изобретение обеспечивает эффективный отбор модифицированных растений без дополнительных молекулярно-биологических тестов. 6 ил., 2 пр.