METHOD FOR DETERMINING DNA OF NODULAR DERMATITIS VIRUS (LSDV) IN BIOLOGICAL MATERIAL OF ANIMALS BY PCR WITH ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS IN AGAROSE GEL

FIELD: biotechnology.SUBSTANCE: invention relates to the field of biotechnology. Invention is a method for determining DNA of nodular dermatitis virus (LSDV) in biological material of animals by PCR with electrophoretic detection of amplification products in agarose gel, involving DNA isolation of infectious disease agent from biological material of infected animal by sorption method, polymerase chain reaction with fluorescent detection with 40 cycles of amplification using oligonucleotide primer DNA-specific DNA infectious agent causative agent DNA, fluorescent dye, an internal control sample in form of suspension of bacteriophage T4 with concentration of 5 × 10phage particles per 1 mcl and a positive control sample in the form of a mixture containing in the same volume ratio fragments of genomes of the causative agent of the infectious animal disease and bacteriophage T4, according to the invention, DNA of the nodular dermatitis virus (LSDV) is recovered in the biological material of animals, performing electrophoretic detection of the amplification products in the agarose gel and obtaining an electrophoregram of DNA with specific bands of the amplified product, visualizing their colouring with a fluorescent dye, according to which recording and interpretation of the PCR results is carried out by the presence or absence of a specific strand of a DNA fragment of the nodular dermatitis virus, coinciding in size with the band of the fragment of the same DNA, amplified from a positive control sample, wherein a mixture of recombinant plasmid DNA containing a DNA fragment of the nodular dermatitis virus and a fragment of the bacteriophage T4 genome with the following nucleotide sequences is used.EFFECT: invention widens functional capabilities in detecting a residual amount of DNA of nodular dermatitis virus.1 cl, 6 tbl Изобретение относится к области биотехнологии. Изобретение представляет собой способ определения ДНК вируса нодулярного дерматита (LSDV) в биологическом материале животных методом ПЦР с электрофоретической детекцией продуктов амплификации в агарозном геле, включающем выделение ДНК возбудителя инфекционного заболевания из биологического материала инфицированного животного сорбционным методом, постановку полимеразной цепной реакции с флуоресцентной детекцией с проведением 40 циклов амплификации с использованием специфичных для участка генома ДНК возбудителя инфекционного заболевания животного олигонуклеотидных праймеров, флуоресцентного красителя