RECOMBINANT YEAST STRAIN PICHIA PASTORIS - PRODUCER OF XYLANASE

FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology. Disclosed is a recombinant yeast strain Pichia pastoris VKPM Y-4393 producing xylanase. Said strain contains a xyl gene which codes endo-1,4-β-xylanase from Paenibacillus brasilensis.EFFECT: strain produces an enzym...

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Hauptverfasser:	Kalinina Anna Nikolaevna, Sineokij Sergej Pavlovich, Gordeeva Tatyana Leonidovna, Borshchevskaya Larisa Nikolaevna, Agranovich Anneta Mikhajlovna
Format:	Patent
Sprache:	eng ; rus
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	FIELD: biotechnology.SUBSTANCE: invention relates to microbiology and biotechnology. Disclosed is a recombinant yeast strain Pichia pastoris VKPM Y-4393 producing xylanase. Said strain contains a xyl gene which codes endo-1,4-β-xylanase from Paenibacillus brasilensis.EFFECT: strain produces an enzyme in amount of 1114 units/ml of culture fluid.1 cl, 3 dwg, 2 ex Изобретение относится к микробиологии и биотехнологии. Предложен рекомбинантный штамм дрожжей Pichia pastoris ВКПМ Y-4393, продуцирующий ксиланазу. Указанный штамм содержит ген xyl, кодирующий эндо-1,4-β-ксиланазу из Paenibacillus brasilensis. Штамм продуцирует фермент в количестве 1114 ед./мл культуральной жидкости. 3 ил., 2 пр.